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. 2020 Apr 8;13:3025–3037. doi: 10.2147/OTT.S237559

Figure 7.

Figure 7

PCAT6 downregulation promoted the radiosensitivity of TNBC cells via sponging miR-185-5p to downregulate TPD52 expression. (A) The protein expression of TPD52 was explored through Western blot analysis in MB-468 and MDA-MB-231 cells transfected si-NC, si-PCAT6, si-PCAT6 + anti-NC, or si-PCAT6 + anti-miR-185-5p. (B) Cell viability was assessed by CCK-8 assay in MDA-MB-468 and MDA-MB-231 cells transfected with si-NC, si-PCAT6, si-PCAT6 + anti-NC, or si-PCAT6 + anti-miR-185-5p under irradiation. (C) Flow cytometry analysis was applied to detect the apoptosis rate in MDA-MB-468 and MDA-MB-231 cells transfected with si-NC, si-PCAT6, si-PCAT6 + anti-NC, or si-PCAT6 + anti-miR-185-5p and then treated with 4Gy irradiation. (D) Colony formation assay was performed to establish clonogenic survival curves in MDA-MB-468 and MDA-MB-231 cells transfected si-PCAT6 + anti-NC or si-PCAT6 + anti-miR-185-5p and then exposed to different doses of irradiation. *P<0.05.