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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: Int J Radiat Oncol Biol Phys. 2020 Jan 25;107(1):194–201. doi: 10.1016/j.ijrobp.2020.01.007

Fig. 3.

Fig. 3.

γH2AX foci in NSC11 xenografts and in vitro radiosensitivity of NSC11 glioblastoma stem-like cells isolated from olfactory bulb and right hemisphere. On day 35 postimplant of NSC11 cells, mice received 6 Gy and were collected for analysis at the indicated time points. (A) Representative images of γH2AX foci in tumor cells (SOX2+) (40 × magnification). The last column corresponds to the overlay used for automated foci counting. (B) γH2AX foci in the corpus callosum, striatum, and olfactory bulb (OB) as a function of time after 6 Gy. Each column corresponds to an individual mouse, with at least 50 cells analyzed per mouse at each location. Bars represent mean ± standard error of the mean. (C). Two NSC11 tumor-bearing mice were euthanized on day 35 postimplant. Green florescent protein fluorescent tissue was collected from the OB and right hemisphere from each mouse, disaggregated, and seeded into standard tissue culture plates in stem cell media. The resulting neurospheres grown from each location were subjected to in vitro clonogenic survival analysis. Values represent the mean ± standard deviation of 3 independent experiments.