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. 2019 Dec 3;18(5):1307–1316. doi: 10.1111/pbi.13296

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© 2019 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Analysis of edits at ZFN‐IPK1 target site in ELS regenerated from transfected microspores. a – the bar graphs represent the percentage of reads with targeted indels after background correction (BC) at subgenome A (sgA) analysed using NGS; b – representative indels at the IPK1‐sgA site in ELS regenerated from microspores transfected with ZFN‐cys‐cys‐CPP. Multiple sequence alignment of the processed reads was done using MAFFT aligner tool in Unipro UGENE program (Okonechnikov et al., 2012). Ct – no transfection control. All treatments were done in three biological replicates.