Figure 4.
Protein–protein interactions between TaDA1‐A and TaGW2‐B. (a) Yeast two‐hybrid assay showing the interaction between TaDA1‐A and TaGW2‐B. BD, GAL4 DNA binding domain; AD, GAL4 activation domain. The yeast strains were serially diluted (100–10‐3) before spotting on selection medium. SD/‐Trp/‐Leu, synthetic dextrose medium lacking Trp and Leu; SD/‐Trp/‐Leu/‐His/‐Ade, synthetic dextrose medium lacking Trp, Leu, His and Ade. (b) LCI assay showing that TaDA1‐A physically interacts with TaGW2‐B in tobacco cells. nLUC, N‐terminal part of LUC; cLUC, C‐terminal part of LUC. The nLUC and cLUC derivative constructs were transformed into A. tumefaciens and then co‐infiltrated into tobacco (N. benthamiana). The LUC signals were collected 48–72 h after infiltration. (c) In vitro pull‐down assay showing that MBP‐TaDA1‐A, but not MBP itself, can pull down the His‐TaGW2‐B protein.