Fig. 3:

Effects of SFN on transcriptional levels of specific genes involved in EMT. Total RNA was extracted from MDA-MB-231 cells treated for 24, 48, and 72 h with 10, 20, 30 and 40μM of SFN. Various concentrations of SFN have different effects on the expression of EMT markers of ZEB1, fibronectin, and claudin-1 after 72 h. Fold changes in gene expression were analyzed relative to gene expression in untreated controls. Significance was set at ^*P<0.05 and ^**P<0.01