Fig. 2.

The NAC conformation of DBE in LinBwt viewed from the main tunnel (left) and from the p3 tunnel (right). As a small molecule, DBE does not interact with many residues besides the catalytic pentad D108, H272, N38, W109 and E138 (not within 4 Å to DBE and thus not shown). DBE is shown as spheres and residues within 4 Å from DBE and the mutated residues are shown as sticks. The mutated residues in the variants follow the color scheme presented in Fig. 1. DBE is shown with the following color coding: brown = bromine, cyan = carbon and white = hydrogen. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)