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. 2020 Apr 3;10:387. doi: 10.3389/fonc.2020.00387

Figure 4.

Figure 4

The role of miR-7977 in regulating lung adenocarcinoma cell proliferation, migration, and invasion. After transfection with miRNA mimics and negative control at final concentrations of 50 nM for 24 h: (A) the expression levels of miR-7975 and miR-7977 in NCI-H1650 and A549 cells were detected using qRT-PCR; (B) the proliferation assay of NCI-H1650 and A549 cells was performed using CCK-8 kit at three time points: 12, 24, and 48 h; (C) wounds were made in NCI-H1650 and A549 cells layer using a sterile micropipette tip, and the width of the wound gap was viewed under a microscope and photographed at 0, 9, and 24 h after wounding; (D) the invasion assay of NCI-H1650 and A549 cells was performed using Matrigel-coated transwell chambers at another 48 h of incubation. The data were expressed as mean ± SD. Each experiment was performed at least three times independently on different days. *P < 0.05, **P < 0.0001. mimic NC, mimic negative control; SD, standard deviation.