Transporter [a] |
Mammalian transporters |
Sodium iodide symporter (NIS, SLC5A5); Norepinephrin transporter (NET, SLC6A2); Dopamine transporter (DAT, SLC6A3). |
Various radiotracers for PET and SPECT for all reporters listed. |
(Dai et al., 1996; Moroz et al., 2007; Jauregui-Osoro et al., 2010; Khoshnevisan et al., 2016, 2017; UCL Business PLC, 2017; Jiang et al., 2018) |
|
Ion transporter from magnetotatic bacteria |
MS-1 magA. |
MRI (Endogenous or exogenous iron). |
(Nakamura et al., 1995; Zurkiya et al., 2008; Cho et al., 2014) |
|
Polypeptides |
Sodium-Taurocholate Co-transporting Polypeptide (NTCP). |
Fluorescence and MRI. |
(Wu et al., 2019) |
Cell surface protein binding [b] |
G-protein-coupled receptors |
Somatostatin receptor type 2 (SSTR2); Dopamine receptor (D2R). |
PET and SPECT radiotracers available; PET radiotracers available. |
(Satyamurthy et al., 1990; MacLaren et al., 1999; Rogers et al., 1999, 2000; Zinn et al., 2000a; Chaudhuri et al., 2001; Liang et al., 2001; Hwang et al., 2007) |
|
Recycling receptor |
Transferrin receptor. |
MRI (SPIO). |
(Weissleder et al., 2000) |
|
Cell-surface antigen-based reporter |
Human carcino-embryonic antigen-based reporters are recombinant proteins based on CEA minigene (N-A3) fused to extracellular and transmembrane domains of human FcγRIIb receptor, CD5 or TfR carboxyterminal domain. |
PET and SPECT radiotracers available. |
(Hammarstrom, 1999; Hong et al., 2008; Kenanova et al., 2009; Barat et al., 2011; Girgis et al., 2011) |
|
Mammalian cell surface protein |
PSMA and mutants; radiotracers bind to the protein using it as a cell surface protein and not exploiting its enzymatic properties. |
PET and SPECT radiotracers available. |
(Castanares et al., 2014; Minn et al., 2019) |
Enzymes [ci] |
Bacterial enzymes |
E. coli dihydrofolate reductase (eDHFR); E. coli β-galactosidase. |
PET; Various including OPTICAL (chemiluminescence), MRI, PET and SPECT. |
(Fowler and Zabin, 1977; Louie et al., 2000; Li et al., 2007; Liu and Mason, 2010; Green et al., 2017; Sellmyer et al., 2017, 2019; Guo et al., 2019; Krueger et al., 2019) |
|
Mammalian and non-viral kinases |
Pyruvate kinase M2, thymidine kinases (viral such as HSV1-tk and mammalian variants), deoxycytodine kinases. |
Various PET tracers for the individual kinases. |
(Tjuvajev et al., 1995; Ponomarev et al., 2007; Jang et al., 2010, 2012; Likar et al., 2010; Park et al., 2015; Lee et al., 2017; Haywood et al., 2019; Seo et al., 2019). |
|
Other mammalian enzymes |
Tyrosinase |
PAT/MSOT, MRI, PET. |
(Weissleder et al., 1997; Ponomarev et al., 2004; Krumholz et al., 2011) |
|
Luciferases |
Various luciferases including Firefly, Green Click Beetle; Gaussia, Renilla; and NanoLuc. |
OPTICAL (bioluminescence): Firefly, Green Click Beetle: D-luciferin; Gaussia, Renilla: coeloenterazine; NanoLuc: imidazopyrazinone. |
(Lorenz et al., 1991; Loening et al., 2006; Tannous, 2009; Inoue et al., 2011; Hall et al., 2012; Schaub et al., 2015; Germain-Genevois et al., 2016; La Barbera et al., 2017; Mezzanotte et al., 2014, 2017; Hunt et al., 2016; Aswendt et al., 2019; Weihs and Dacres, 2019; Zhang et al., 2019) |
Fluorescent Proteins [cii] |
Proteins with intrinsic fluorophores |
Red fluorescent: E2-Crimson/mTagRFP/mPlum/mNeptune; Infrared fluorescent: iRFP 670/iRFP 720. |
OPTICAL (fluorescence upon appropriate excitation): (emission λmax): 543/584/649/650; (emission λmax): 670/720. |
(Merzlyak et al., 2007; Kremers et al., 2009; Lin et al., 2009; Filonov et al., 2011; Liu et al., 2013; Shcherbakova and Verkhusha, 2013; Deliolanis et al., 2014; Isomura et al., 2017; Zhou et al., 2018; Fukuda et al., 2019) |
Frequency-selective contrast/other |
Artificial protein |
Contrast based on transfer of radiofrequency labeling from the reporter’s amide protons to water protons. |
MRI (CEST). |
(Gilad et al., 2007; Farrar et al., 2015) |
Formation of gas vesicles/other |
Mammalian acoustic reporter gene (mARG) |
Gas vesicles are produced which generate US contrast. |
US (3.2 MPa insonation). |
(Farhadi et al., 2019) |