Fig. 3.

Two-dimensional thiol proteome analysis of NRVM. SCR and frataxin-deficient cultures were processed and analyzed as described in material and methods. Representative analytical gels containing SCR and shFxn samples are shown in A (Bodipy signal, which corresponds to oxidized thiol groups) and B (Flamingo signal, which corresponds to total protein stain). The indicated spots are those showing significant differences in their ratio Bodipy/flamingo; C, amplified image of the gel region containing spots 1 and 2; D, histograms show the relative Bodipy/protein ratio from spots 1 and 2. Data are mean +/- SEM from three independent experiments.