Fig. 6.

Protein content analysis by targeted proteomics. A, a representative chromatogram showing the SRM traces of the different peptides analyzed in the targeted proteomics experiment. B, representative signals from the three transitions detected for the peptide AILAELTGR, corresponding to PDH A1. Top panel corresponds to the detection of the “light” version of the peptide obtained from the tryptic digestion of whole cells lysates, while low panel corresponds to the detection of the standard, a C13N15 labeled “heavy” version of the peptide. C, Histograms show the relative content (in arbitrary units) of the measured proteins by targeted proteomics. Data are represented as means ± SEM from 4 independent experiments. * indicates significant (p<0.05) differences between shSCR and shFxn conditions.