Fig. 9.

Tiron supplementation prevents lipid droplet accumulation. NRVM cultures were supplemented with the indicated concentrations of Tiron and, after 7 days of frataxin depletion, lipid droplets were stained with Bodipy 493/503 (A). The number of lipid droplets per cell was measured with ImageJ (B). Graphs represent the relative number of lipid droplets relative to non-treated FXN deficient cells values. C, Histograms show the relative content (in arbitrary units) of the measured proteins by targeted proteomics. Data are represented as means ± SEM from 3 independent experiments. *(p<0.05) and ** (p<0.01) indicate significant differences between shSCR and shFxn conditions. # indicates statistic significance to non-treated shFxn (p<0,05).