Table III.
LC–MS-MS Method Development Metrics
| Analyte | Internal standard | Ionization mode | Transitions (± 0.3 amu) |
Retention time (± 0.3 min) |
|---|---|---|---|---|
| Enzyme hydrolysis assay | ||||
| 8,11-diOH-∆9-THC | Positive | 347.3 > 311.4 347.3 > 293.3 |
2.3 | |
| 8,11-diOH-∆9-THC-D6 | Positive | 353.3 > 317.4 353.3 > 299.3 |
2.3 | |
| 11-OH-THC | Positive | 331.2 > 193.1 331.2 > 201.1 |
4.6 | |
| 11-OH-THC-D3 | Positive | 334.2 > 196.1 334.2 > 201.1 |
4.6 | |
| THCV | Positive | 287.3 > 165.1 287.3 > 231.1 |
5.5 | |
| CBD-D3 | Positive | 318.2 > 196.2 318.2 > 123.2 |
7.3 | |
| CBD | Positive | 315.2 > 193.2 315.2 > 123.2 |
5.8 | |
| CBD-D3 | Positive | 318.2 > 196.2 318.2 > 123.2 |
5.8 | |
| CBN | Positive | 311.1 > 223.1 311.1 > 241.0 |
7.3 | |
| CBN-D3 | Positive | 314.1 > 223.1 314.1 > 241.0 |
7.3 | |
| ∆9-THC | Positive | 315.1 > 193.2 315.1 > 259.2 |
8.0 | |
| ∆9-THC-D3 | Positive | 318.1 > 196.2 318.1 > 262.2 |
8.0 | |
| ∆8-THC | Positive | 315.1 > 193.2 315.1 > 259.2 |
8.2 | |
| ∆8-THC-D9 | Positive | 324.1 > 202.2 324.1 > 268.2 |
8.2 | |
| Base hydrolysis assay | ||||
| 8-β-OH-∆9-THC | Positive | 331.2 > 201.1 331.2 > 271.1 |
3.5 | |
| 11-OH-THC-D3 | Positive | 334.2 > 196.1 334.2 > 201.1 |
5.1 | |
| THCVA | Negative | 315.2 > 217.1 315.2 > 163.1 |
3.05 | |
| ∆8-THCCOOH-D6 | 349.1 > 251.1 349.1 > 191.1 |
5.25 | ||
| ∆8-THCCOOH | Negative | 343.1 > 245.1 343.1 > 191.1 |
5.25 | |
| ∆8-THCCOOH-D6 | Negative | 349.1 > 251.1 349.1 > 191.1 |
5.25 | |
| ∆9-THCCOOH | Negative | 343.1 > 245.1 343.1 > 191.1 |
5.45 | |
| ∆9-THCCOOH-D9 | Negative | 352.1 > 254.1 352.1 > 194.1 |
5.45 |