Fig. 2.

Increase of TG, perilipin, and PPARγ production by insulin in hamster preadipocytes. A and B: TG production was increased by insulin (0.1–20 nM) in dose- and time-dependent manners, respectively. C: When hamster preadipocytes, after the initial stimulation with Dex and IBMX were treated every two days for ten days with or without insulin (20 nM), the production of perilipin was augmented by insulin. However, there were no changes in the constitutive expressions of PPARγ1 and PPARγ2 between the insulin-untreated and -treated cells. Three independent experiments were reproducible and typical findings are shown. * and ***, significantly different from untreated cells (Cont) or day 0 (p < 0.05 and 0.001, respectively).