Table 1.
Accurate monomer masses | ||
---|---|---|
Protein, z+ | Sequence mass (Da) | Measured mass (average ± S.D., Da) |
QT2–4, 4–7+ | 8592.37 | 8645.84 ± 0.05 |
LC8, 3–7+ | 10,770.31 | 10,638.52 ± 0.20 |
Masses of QT2–4/LC8 species | ||
Species/complex, z+ | Expected mass (Da) | Measured mass (average ± S.D., Da) |
LC8 monomer, 3–8+ | 10,638.52 ± 0.20 | 10,753 ± 2 |
LC8 dimer, 8–10+ | 21,277.0 ± 0.4 | 21,503 ± 1 |
QT2–4 monomer + 1 LC8 dimer | 29,922.9 ± 0.4 | 30,154.2 ± 0.9 |
QT2–4 monomer + 2 LC8 dimers | 51,200 ± 1 | 51,765 ± 8 |
QT2–4 dimer + 3 LC8 dimers | 81,123 ± 1 | 81,925 ± 21 |
QT2–4 dimer + 4 LC8 dimers | 102,400 ± 2 | 103,497 ± 15 |