Figure 3.

Conformational diversification of Wisc-1 prions in deer expressing PrP^C polymorphisms Ser-96 and His-95. Structural stability of deer PrP^CWD after exposure to increasing concentrations of GdnHCl (m). The apparent fractional change (F_app) of unfolding was measured by CDI with 8G8 or 12B2 mAbs before or after proteinase K treatment (PK −/+). CWD conformers encoded in all three PrP primary structures were detected with 8G8 mAb, whereas 12B2 only recognized prions encoded on WT–PrP. Black dotted lines indicate the denaturation midpoints (GdnHCl_1/2). F_app values and bars represent the mean ± S.E. obtained for each individual deer CWD lineage (two batches of 20% w/v brain homogenate) measured in triplicate with each Eu-labeled mAb.