Figure 4.

Sedimentation properties and protease resistance of PrP^CWD from Wisc-1 deer CWD lineages. Fractions collected from the bottom of the tube were analyzed by Western blotting and CDI prior (−) or after (+) treatment with proteinase K. A, distribution of total and PK-res WT–PrP^CWD in different CWD allotypes detected with 12B2 mAb. B, concentration of fractionated WT–PrP^C (green), total WT–PrP^CWD (orange), and PK-res WT–PrP^CWD (black) was determined for WT/WT, Ser-96/WT, and His-95/WT prions using CDI with 12B2 mAb. Detection of His-95 and Ser-96 PrP^CWD required 8G8 mAb. Bars represent average ± S.E. CDI measurements were performed on each individual deer sample in triplicate.