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. 2020 Mar 9;295(15):4937–4949. doi: 10.1074/jbc.RA119.010498

Figure 4.

Figure 4.

Knockdown of MyHC IIA/X-AS impairs the myogenic differentiation in porcine skeletal satellite cells. A, representative immunofluorescence results for MyHC-positive myotubes 6 days post-myogenic differentiation upon transfection with either MyHC IIA/X-AS siRNA or negative control. Scale bar = 1 mm. n = 3. B, statistical results of differentiation index. The differentiation index was calculated as the percentage of MyHC-positive nuclei among total nuclei (n = 3). C, statistical results of myotube fusion index (n = 3). Myotube fusion index was determined as the distribution of nucleus number in total myotubes. D, mRNA levels of myogenic-related genes were detected through qRT-PCR in myotubes 6 days post-myogenic differentiation (n = 5). E and F, protein levels of myogenic-related genes were detected by Western blotting in myotubes 6 days post-myogenic differentiation (n = 3). NC, negative control. *, p < 0.05; **, p < 0.01.