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. 2020 Mar 9;295(15):4937–4949. doi: 10.1074/jbc.RA119.010498

Figure 5.

Figure 5.

Reduced MyHC IIA/X-AS induces a fast–to–slow switch. A, expression of MyHC IIA/X-AS in different muscles of 180-day-old pigs was tested through qRT-PCR (n = 5). B, mRNA levels of MyHC isoforms were detected through qRT-PCR in myotubes 6 days post-myogenic differentiation transfected with either MyHC IIA/X-AS siRNA or negative control (NC) (n = 3). C and D, changes of MyHC I and IIa at protein levels were estimated through Western blotting (n = 3). E, mRNA levels of muscle fiber marker genes, PGC-1α and FoxO1, were detected through qRT-PCR in myotubes 6 days post-myogenic differentiation. The cells were transfected with either MyHC IIA/X-AS siRNA or negative control (n = 3). F and G, protein levels of PGC-1α and FoxO1 were determined by Western blotting (n = 3). *, p < 0.05; **, p < 0.01.