Figure 7.

Overexpression of miR-130b impedes the myogenic differentiation in porcine skeletal satellite cells. A, representative immunofluorescence staining of MyHC-positive myotubes 6 days post-myogenic differentiation. The cells were transfected with miR-130b agomir or negative control (NC) (n = 3). Scale bar = 1 mm. B, statistical results of differentiation index (n = 3). C, statistical results of fusion index (n = 3). D, changes of myogenic-related genes at mRNA levels after AgomiR-130b transfection were detected through qRT-PCR. Cells were harvested 6 days post-myogenic differentiation (n = 5). E and F, protein levels of myogenic factors were detected by Western blotting 6 days post-myogenic differentiation (n = 3). *, p < 0.05; **, p < 0.01.