Figure 8.

Enforced overexpression of miR-130b drives a fast–to–slow fiber phenotype. A, expression pattern of miR-130b in skeletal muscles of 180-day-old pigs was analyzed through qRT-PCR (n = 5). B, changes of MyHC isoforms at mRNA levels were detected through qRT-PCR in myotubes upon AgomiR-130b transfection 6 days post-myogenic differentiation (n = 5). C and D, changes of MyHC I and IIa at protein levels upon AgomiR-130b transfection were determined by Western blotting in myotubes 6 days post-myogenic differentiation (n = 3). E, mRNA levels of muscle fiber marker genes, PGC-1α and FoxO1, were detected through qRT-PCR in myotubes 6 days post-myogenic differentiation. The cells were transfected with either miR-130b agomir or negative control (NC) (n = 5). F and G, protein levels of PGC-1α and FoxO1 were evaluated by Western blotting (n = 3). *, p < 0.05; **, p < 0.01.