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. 2020 Mar 9;295(15):5081–5094. doi: 10.1074/jbc.RA119.010534

Figure 6.

Figure 6.

A–C, HIV-1 infection does not affect miR-125b precursors. HEK-293T cells were inoculated with pseudotyped HIV-1 GFP reporter virus and levels of (A) pri-, (B) pre-, and (C) mature miR-125b was measured in infected and uninfected cells by qPCR. D and E, down-regulation of miR-125b depends on HIV-1 reverse transcription. D, HEK-293T cells were inoculated with pseudotyped HIV-1 GFP virus in the presence of EFV or RAL and productive infection was assessed by GFP expression. E, RNA from these cells were analyzed for measuring miR-125b levels by qPCR. F-H, effects of miR-125b expression on early steps of HIV-1 infection. HEK-293T cells were transfected with either anti-miRs or mimics of miR-125b and after 24 h, inoculated with pseudotyped HIV-1 GFP virus. RNA from these cells was isolated and subjected to qPCR to measure copies of (F) reverse transcription, (G) 2-LTR circles, and (H) integration. Data presented are from three independent experiments. Error bars represent S.E. *, represents p < 0.05 for the comparison of uninfected versus infected cells.