Figure 5.

Effect of AgNPs on virulence factors of C. albicans. (A) Microscopic examination of the dimorphic transition in RPMI-1640 medium supplemented with either amphotericine b, AgNPs, or AgNPs/CG. Complete inhibition of the dimorphic transition (all cells were observed in yeast form when AgNPs and plant extract were combined, unlike when the two were tested on their own). (B) Percentage of adhesion after (2 h) in RPMI-1640 medium supplemented with 0.25% glucose. (C) Biofilm formation (after 24 h) as measured using an MTT metabolic assay. Values are the mean ± SD of three independent experiments (* p < 0.05, ** p < 0.005).