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. 2020 Feb 27;10(3):418. doi: 10.3390/nano10030418

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Intracellular localisation of C-BNM after 24 h exposure in THP-1 (a–d) and primary monocytes (e,f): GP1 (a,b,e) forms large aggregates in cytoplasm apparently in vesicles. No particles are found in nucleus (N); (c,f) GP2 forms smaller aggregates in cytoplasm, apparently in vesicles. Occasionally, free particles are detected in cytoplasm; (d) MWCNT were found as free needle-like objects in the cytoplasm, possibly from damaged lysosomes. Sporadically, they can be found in nucleus or penetrating through the nuclear membrane (Nm).