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Chromatographic condition (Gradient).
| Time (min) | Solvent A% | Solvent B% | Flow (mL/min) |
|---|---|---|---|
| 0.0 | 70 | 30 | 1 |
| 5.0 | 61 | 39 | 1 |
| 7.0 | 56 | 44 | 1 |
| 10.0 | 54 | 46 | 1 |
| 11.0 | 51 | 49 | 1 |
| 13.0 | 48 | 52 | 1 |
| 15.5 | 47 | 53 | 1 |
| 18.0 | 47 | 53 | 1 |
| 19.8 | 46 | 54 | 1 |
| 19.9 | 41 | 59 | 1 |
| 20.0 | 30 | 70 | 1 |
| 23.9 | 30 | 70 | 1 |
| 24.0 | 70 | 30 | 1 |
| 28.0 | 70 | 30 | 1 |
