Fig. 3.

Lysates of HCT-116 exposed to 5-FU + CQ increase DC maturation and activation. DCs from six healthy donors were exposed to the lysates of HCT-116 cells previously treated with the minimal effective concentrations of 5-FU or its combination with CQ (5-FU + CQ). WT refers to DCs pulsed with the lysate of untreated HCT-116 cells, and CTRL refers to unstimulated DCs. Scatter plots illustrate the percentages of DCs (CD11c+) that co-express the markers CD83 (A), HLA-DR (B), CD80 (C), and CD86 (D). The mean and standard deviation of the six individual donors was analyzed by ANOVA. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.005.