Table 2.
Method validation of NAR in rat plasma (n = 6).
| NAR concentration (ng/mL) | ||||||
|---|---|---|---|---|---|---|
| 1.03 | 3.08 | 410 | 616 | |||
| Precision accuracy | Intraday | RE (%) | 1.4 | −6.2 | 4.7 | 3.4 |
| RSD (%) | 8.3 | 8.5 | 3.2 | 4.3 | ||
| Interday | RE (%) | 2.8 | −2.6 | −0.5 | −1.4 | |
| RSD (%) | 1.7 | 6.2 | 6.9 | 6.3 | ||
|
| ||||||
| Recovery | R (%) | 69.0 | 69.5 | 69.9 | 76.5 | |
| RSD (%) | 9.0 | 6.5 | 3.7 | 10.2 | ||
|
| ||||||
| Matrix effect | ME (%) | 111.4 | 131.2 | 116.9 | 107.7 | |
| RSD (%) | 7.7 | 5.3 | 2.6 | 1.1 | ||
|
| ||||||
| Stability | Short-term (24 h) | RE (%) | 3.5 | 10.3 | 0.9 | 1.7 |
| RSD (%) | 6.9 | 3.2 | 7.8 | 0.5 | ||
| Freeze-thaw cycles | RE (%) | 1.8 | 2.8 | 4.8 | 1.6 | |
| RSD (%) | 11.1 | 12.2 | 3.9 | 2.3 | ||
| Long-term (21 days) | RE (%) | 0.2 | −6.5 | 0.1 | −6.9 | |
| RSD (%) | 6.2 | 10.1 | 5.1 | 2.1 | ||
| Autosampler (24 h) | RE (%) | 2.0 | 4.4 | −8.4 | −8.3 | |
| RSD (%) | 5.4 | 9.3 | 3.6 | 4.0 | ||