Figure 7.

Interactions of the PPARγ and RXRα complex with DEGs of RT-EVCTs and RT-VCTs. Predictions were made of protein-protein interactions between PPARγ and DEG-encoded proteins, as well as of the transcription factor-target gene (TF-TG) interactions of PPARγ with DEG promoters. The PPARγ and RXRα (heterodimeric nuclear receptor partner of PPARγ) complex, together with the DEGs recovered in this study, were submitted to the STRING online tool. Visualizations were modified in Cytoscope to depict hierarchical interactions and gene expression. For hierarchical protein-protein interactions, red text represents upregulated genes, blue text represents downregulated genes, blue circles represent direct interactions with the PPARγ complex, red circles represent second-level interactions, and grey circles represent plus-level interactions. TF-TG interactions of the PPARγ complex with DEG promoters were predicted by iRegulon based on the TRANSFAC database. Red circles represent upregulated DEGs and blue circles represent downregulated DEGs. PPARγ: peroxisome proliferator-activated receptor-γ; RXRα: retinoid x receptor-α; RT-EVCTs: rosiglitazone-treated extravillous cytotrophoblasts; RT-VCTs: rosiglitazone-treated villous cytotrophoblasts; DEGs: differentially expressed genes; PPI: protein-protein-interaction; TF-TG: transcription factor-target gene.