Table 1.

Participants, instruments, and software. Five centres, with a total of 13 operators (including the reference operator, ROP), using seven different flow cytometers dedicated to research use (except GMP-maintained BD FACSCanto™ I at ISS, with a fluidic system upgrade, comparable to a BD FACSCanto™ II), participated to the harmonisation panel. Three flow cytometer models with compatible optical configuration (BC Gallios™, BD FACSCanto™ II, and BD LSRFortessa™) were used. The data generated were analysed by operators at peripheral sites (local analysis) using their own analysis software (Kaluza, FlowJo, or FACSDiva). Central analysis at ISS was performed by the ROP with Kaluza software on local raw data (acquired fcs files).

5 centres	13 operators	7 instruments	3 instrument models	2 acquisition software	3 analysis software
ISS SUR INMI IRE OPBG	12 + 1 ROP	1 FACSCanto I+ (GMP) 3 BD FACSCanto II 2 BD LSRFortessa 1 BC Gallios	BD FACSCanto II BD LSRFortessa BC Gallios	BC Kaluza BD FACSDiva	BC Kaluza BD Diva TreeStar FlowJo