Table 2.
Marker | Fluorochrome | Clone | Sample | Supplier | Format | |
---|---|---|---|---|---|---|
a+b | CD4 | FITC | 13B8.2 | PBMC/WB | Beckman Coulter | DURAClone custom backbone (dried) |
CCR7 (CD197) | PE | G043H7 | PBMC/WB | |||
CD8 | PeCy5.5 | B9.11 | PBMC/WB | |||
CD3 | PeCy7 | UCHT-1 | PBMC/WB | |||
CD45RA | APC | 2H4 | PBMC/WB | |||
| ||||||
a | Dead exclusion marker | Near-IR (NiR) | Live/dead Fixable dead cell stain kit |
PBMCs | Thermo Fisher | Drop-in (liquid) |
| ||||||
b | CD45 | APC-Cy7 | 2D1 | WB | BioLegend | Drop-in (liquid) |