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. 2020 Apr 12;2020:1938704. doi: 10.1155/2020/1938704

Table 2.

Antibody specifications–naïve/memory panel. Antibody-fluorochrome conjugates for naïve/memory T cell phenotype panel. The panel was composed of a backbone dried mixture of five antibodies (a+b) and the drop-in liquid markers: live/dead discriminator for PBMC (a) and anti-CD45 APC-Cy7 for WB (b) samples.

Marker Fluorochrome Clone Sample Supplier Format
a+b CD4 FITC 13B8.2 PBMC/WB Beckman Coulter DURAClone custom backbone (dried)
CCR7 (CD197) PE G043H7 PBMC/WB
CD8 PeCy5.5 B9.11 PBMC/WB
CD3 PeCy7 UCHT-1 PBMC/WB
CD45RA APC 2H4 PBMC/WB

a Dead exclusion marker Near-IR (NiR) Live/dead
Fixable dead cell stain kit
PBMCs Thermo Fisher Drop-in (liquid)

b CD45 APC-Cy7 2D1 WB BioLegend Drop-in (liquid)