Figure 10.

Inhibition of Nrf2 activity partly abolishes the anti-inflammatory, antiapoptotic, and mitochondrial protective role of rhSTC1. HK-2 cells were treated with iohexol (200 mg iodine/ml) for 4 hours with or without rhSTC1 (50 ng/ml) or ML385 (10 μM). (a) Representative images of transmission electron microscope analysis. (b) Western blot analysis of the expression of inflammation, apoptosis, and mitochondrial damage was associated with proteins (n = 3). (c) Western blot analysis of the expression of nuclear and cytoplasm Nrf2 (n = 3). (d–l) Quantification of average Western blot band intensities. Values were presented as mean ± SE. ^∗p < 0.05, compared with the control group. ^#p < 0.05, compared with the iohexol group. ^▲p < 0.05, compared with the rhSTC1 treatment group.