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. 2020 Apr 12;2020:4794982. doi: 10.1155/2020/4794982

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Copyright © 2020 Zhenhui Liu et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Proliferation of RSC96 cells. (a) Observation of RSC96 cell proliferation in different culture conditions under a phase contrast microscope. (b) RSC96 cell proliferation measured by CCK8 assay, and the result showed that the scaffolds with 0.05% MWCNT and 0.1% MWCNT demonstrated cytotoxicity on day 3 and day 5 (^∗P < 0.05). (c) showed the neurofilament images of RSC96 cells cocultured with scaffolds by immunofluorescence. Scale bar = 50 μm. (d) showed the relative expressions of NF through cell immunofluorescence intensity on scaffolds with different concentration of MWCNTs and compared with the control scaffolds after cultured for 3 days. No difference was found between any two scaffolds.