Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 9;11:2041731420911816. doi: 10.1177/2041731420911816

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

PMC Copyright notice

Figure 6. — Final vascularization of MVF-seeded matrices. (a–d) Immunohistochemical detection of CD31⁺ microvessels within MVF-seeded matrices in a PBS- (a, c) and a clo-treated (b, d) mouse (a, b = overview of the implants; c, d = higher magnification of inserts in a and b; broken lines = implant borders; arrows = CD31⁺ microvessels). Scale bars: a, b = 230 µm; c, d = 45 µm. (e) Microvessel density (mm⁻²) within MVF-seeded matrices in PBS- (control; white bar; n = 8) and clo-treated (black bar; n = 8) mice. Means ± SEM. *p < 0.05 vs control. (f–h) Representative immunohistochemical detection of CD31⁺/GFP⁺ microvessels (arrows) and CD31⁺/GFP⁻ microvessels (arrowheads) within a MVF-seeded matrix of a clo-treated mouse. Scale bars: 30 µm. (i) GFP⁺ microvessels (%) within MVF-seeded matrices in PBS- (control; white bar; n = 8) and clo-treated (black bar; n = 8) mice. Means ± SEM. (j, k) Immunohistochemical detection of CD31⁺ microvessels with (arrows) and without (arrowheads) a perivascular α-SMA⁺ cell layer within MVF-seeded matrices of a PBS- (j) and a clo-treated (k) mouse. Scale bars: 18 µm. (l) α-SMA⁺ microvessels (% of all blood vessels) within MVF-seeded matrices in PBS- (control; white bar; n = 8) and clo-treated (black bar; n = 8) mice. Means ± SEM. *p < 0.05 vs control.