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. 2020 Feb 26;245(7):620–630. doi: 10.1177/1535370220908041

Figure 2.

Figure 2.

The functions of KCNQ1OT1 on the viability and apoptosis of LPS-induced H9c2 cardiomyocytes. (a) qRT-PCR validated the overexpression of KCNQ1OT1 and its change when treated with LPS in H9c2 cells. (b) qRT-PCR detected KCNQ1OT1 expressions in H9c2 cells at different LPS concentrations. (c) qRT-PCR was adopted to detect KCNQ1OT1 expressions in H9c2 cells at different treatment times. (d) CCK-8 assay was employed to evaluate the viability of LPS-induced H9c2 cardiomyocytes after KCNQ1OT overexpression. (e) The apoptosis of LPS-induced H9c2 cells after KCNQ1OT overexpression was detected. (f) Western blot was conducted to detect caspase-3 and Bax after KCNQ1OT overexpression in H9c2 cells. ( g–i) ELISA was used to detect TNF-α, IL-1β and IL-6 expressions in the supernatant of cells. v.s. control group: *, **, *** indicated P < 0.05, P < 0.01, P < 0.001, respectively. v.s LPS group: &, &&, &&& indicated P < 0.05, P < 0.01, P < 0.001, respectively.