The expression of spermatogenesis-specific genes in the adult 6-month-old mice. (A–C) Real time PCR of tisp69, tisp76, ar, sox9, vimentin, cx43, sprm1, prm1, and elovl2, 4, 5, and 6 in the cRNA of the testis. Fold expression of control in the (A) nd-, (B) AA-, and (C) DHA-fads2−/− males normalized to wt-nd, -AA, and -DHA. (D–F) Western Blotting analysis of the junction complex proteins in lysates of the nd-, AA-, and DHA-fads2−/− testes using (D) anti-Cx43, (E) anti-Claudin 11, and (F) anti-Occludin antibody.