Figure 4.

Inhibition of EWS-FLI1 expression in tumor xenografted on mice treated for 24 h by siRNA vectorized by tritiated DND (T-DND) at a mass ratio of 5:1 (T-DND:siRNA). The tumor was formed from A673 Ewing sarcoma cells grafted in the right flank of the mice (n = 6 to 8 animals per condition). T-DND:siRNA was intravenously injected in the mouse tail vein. RNA was extracted from the tumor of mice sacrificed 24 h after treatment, and RT-qPCR was performed using GAPDH as housekeeping gene. Standard error on the mean are indicated. siAS:siRNA against EWS-FLI1 oncogene; siCt: irrelevant. A significant difference between T-DND:siAS and T-DND-siCt is observed (*) according to the p_Dunn value <0.05, inferred from a Kruskal–Wallis test (p = 0.0094) followed by a Dunn multiple comparisons test.