Figure 5.

Mouse organ distribution of uncomplexed T-DND or complexed with siRNA at mass ratio 5:1 of T-DND:siRNA. The total radioactivity of each tissue is measured after 4 h and 24 h for 3 animals per series. Error bars: standard deviations. (a) Radioactivity presented as count per minutes (cpm) per gram of tissue or feces. The latter corresponds only to the fraction collected in the rectum of each animal. (b) Radioactivity from each full organ, presented as the percentage of the total injected dose. Feces were collected for all the animals simultaneously in the same metabolism cage, but the total amount, including non-expelled feces is not known which is why we could not include feces data in B. ND + siAS: DND complexed with siRNA sequence antisense for EWS-FLI1 oncogene. The differences between error bars for the different conditions are due to (i) the variability between animal groups, and (ii) to the fact that the T-DND quantifications were done only on portions of ≈100 mg of each organ, and that we cannot exclude an inhomogeneous distribution of T-DND within the organs.