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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Breast Cancer Res Treat. 2019 Nov 19;179(3):585–604. doi: 10.1007/s10549-019-05485-5

Fig. 6. ERRβ isoform expression by IHC.

Fig. 6.

A-D. Automatic scanning and semi-automatic quantification of ERRβ splice variant specific mouse monoclonal antibodies (ab) on tissue microarray (TMA). a. Blue/brown staining with ERRβ ab. Automatic detection of b. nuclear v cytoplasmic staining, and c. cells “positive”/ above threshold vs. “negative”/ below threshold. d. ERRβ protein expression in breast tumors. Representative images of ER+, HER2, and TNBC tumor tissues from 150 patient tissue microarray series stained with ERRβ ab