Table I.

Multiexponential Analysis of Cy3-DNA and Cy5-DNA Intensity Decays

Compound/Conditions	τ (ns)	〈τ〉 (ns)	α1 (f1)	τ1 (ns)	α2 (f2)	τ2 (ns)	α3 (f3)	τ3 (ns)	${\chi }_{\mathrm{R}}^2$
Cy3-DNA, quartz before photobleaching	1.21a)	0.90b)	0.433 (0.138)	0.29	0.567 (0.862)	1.36	–	–	1.7
After photobleaching	1.25	1.03	0.272 (0.064)	0.26	0.728 (0.933)	1.32	–	–	1.9
Cy3-DNA, silver before photobleaching	0.78	0.141	0.860 (0.285)	0.047	0.103 (0.306)	0.42	0.037 (0.409)	1.57	2.0
After photobleaching	0.62	0.063	0.906 (0.316)	0.030	0.066 (0.265)	0.34	0.028 (0.419)	1.25	2.5
Cy5-DNA, quartz before photobleaching	1.53	1.20	0.382 (0.127)	0.40	0618 (0.873)	1.69	–	–	2.5
After photobleaching	1.50	1.19	0.354 (0.110)	0.37	0.646 (0.890)	1.64	–	–	2.4
Cy5-DNA, silver before photobleaching	0.99	0.016	0.989 (0.390)	0.006	0.006 (0.064)	0.17	0.05 (0.546)	1.79	1.1
After photobleaching	0.76	0.002	0.9995 (0.575)	0.001	0.0001 (0.024)	0.34	0.004 (0.401)	1.88	1.3

^a$\overline{\tau }={\sum }_i{f}_i{\tau }_{\mathrm{i}}$, $f_i=\frac{{\alpha }_i{\tau }_i}{\sum {\alpha }_i{\tau }_i}$

^b$\langle \tau \rangle ={\sum }_i{\alpha }_i{\tau }_i$