Figure 3.

MBD-seq and WGB-seq provide comparable methylome profiles.

Difference (Δ) in methylation between neurons and glia along chromosome 17. Methylation was assayed in sorted neurons and glia from a human brain sample by WGB-seq, MBD-seq, and MeDIP-seq. Positive values indicate higher methylation in neurons. (a) Chromosome-wide trace of neuron-glia differences in methylation along chr17. To facilitate visualization the 10 kb simple moving average of Δ methylation is plotted. Highlighted region indicates the location of the enlarged region displayed in the lower pane. (b) Enlarged region along chr17:77,090,000–77,096,500 (hg19) displaying focal Δ methylation between neurons-glia. Highlighted region indicates the location of cell-type-specific differential methylation that is detected in WGB-seq and MBD-seq, but not MeDIP-seq. Locations of Infinium HumanMethylation 450K sites are also shown for illustration.