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. 2019 Oct 10;15(4):386–397. doi: 10.1080/15592294.2019.1676613

Figure 5.

Figure 5.

Effect of miR-99b-5p on target gene expression.

(a). Relative mRNA levels of 7 target genes in miR-99b-5p OE cells and OE-NC cells using RT-qPCR. The expression was normalized against glyceraldehyde-3-phosphate dehydrogenase (GAPDH). (b). Relative mRNA levels of 2 target genes in human PBMC in 35 RA cases and 35 controls using RT-qPCR. The expression was normalized against beta-2-microglobulin (B2M). (c). Dual luciferase assay of Jurkat T cells co-transfected with the luciferase reporter plasmid containing the wild-type or mutant-type RASSF4-3ʹUTR and miR-99b-5p overexpression lentivirus or negative vector. Two-sided Student’s t-test was used for the comparisons between groups. * P < 0.05, ** P < 0.01, *** P < 0.001. OE: over-expression cells, OE-NC: negative control cells.