Figure 5. Transcription activation analysis of ZmHsf01 in yeast.
Yeast cells transformed with pGBKT7 vector were set as the negative control, and yeast cells co-transformed with pGBKT7-53 and pGADT7-T vectors were set as the positive control. The ZmHsf01 group were the yeast cells transformed with the fusion vector pGBKT7-ZmHsf01. (A) The negative control in the SD/Trp- culture medium. (B) The negative control in the SD/Trp-/His-/Ade-/X-α-gal culture medium. (C) The positive control in the SD/Trp- culture medium. (D) The positive control in the SD/Trp-/His-/Ade-/X-α-gal culture medium. (E) The fusion vector pGBKT7-ZmHsf01 in the SD/Trp- culture medium. (F) The fusion vector pGBKT7-ZmHsf01 in the SD/Trp-/His-/Ade-/X-α-gal culture medium.