Fig. 3.

Trastuzumab internalization rate determined for different breast cancer cells. (A) Bright green color in each image represents cell membrane bound and internalized fraction of Alexa Fluor 488–conjugated trastuzumab. Each image in the panel represents one cell out of 1500 gated cells that were used for calculation at each time point. (B) The plot of internalization score vs. time used to calculate internalization half-life. Solid square, upward triangle, and downward triangle represents SKBR-3, MDA-MB-453, and MCF-7 cells, respectively. Symbol represents mean and error bars represent S.D.