Skip to main content
. 2020 Apr;48(4):297–306. doi: 10.1124/dmd.119.090043

TABLE 1.

Sequences of oligonucleotides and primers

Name Sequence (5′—3′)
For quantitative real-time PCR
LINC00574 forward CGG AGA AGC TCC TCT TGT GA
LINC00574 reverse GCC CAG TAC TCT CCT GGA TG
UGT2B15 forward GAA AAT TCT CGA TAG ATG GAT ATA TGG TG
UGT2B15 reverse AAC TGC ATC TTT ACA GAG CTT GTT ACT G
GAPDH forward GAA ATC CCA TCA CCA TCT TCC AGG
GAPDH reverse GAG CCC CAG CCT TCT CCA TG
hsa-miR-129-5p forward CUU UUU GCG GUC UGG GCU UGC
miRNA Universal reverse AAC GCT TCA CGA ATT TGC GT
U6 forward CGC TTC GGC AGC ACA TAT AC
U6 reverse AAA ATA TGG AAC GCT TCA CGA
For LINC00574 knockdown
Negative control ASO CGA CUA TAC GCG CAA UAU GG
LINC00574 ASO GUG ACA GCA AAC TAC CGA AC
LINC00574 siRNA forward GUC CCU CCU GCC UCA GUU UCU CAG A
LINC00574 siRNA reverse UCU GAG AAA CUG AGG CAG GAG GGA CUG
For luciferase gene reporter assay
LINC00574 PCR forward GGG AAA GUT CTC CCA GCC TAG CCC TAC T
LINC00574 PCR reverse GGA GAC AUA AGG GCG AGT AGC AAG AAC A
For FREMSA
LINC00574 oligo GGC AGA GCU CGG GGU UGC UGC GGA GC
hsa-miR-129-5p oligo CUU UUU GCG GUC UGG GCU UGC
miRNA negative control oligo AAC GCT TCA CGA ATT TGC GT