For quantitative real-time PCR |
|
LINC00574 forward |
CGG AGA AGC TCC TCT TGT GA |
LINC00574 reverse |
GCC CAG TAC TCT CCT GGA TG |
UGT2B15 forward |
GAA AAT TCT CGA TAG ATG GAT ATA TGG TG |
UGT2B15 reverse |
AAC TGC ATC TTT ACA GAG CTT GTT ACT G |
GAPDH forward |
GAA ATC CCA TCA CCA TCT TCC AGG |
GAPDH reverse |
GAG CCC CAG CCT TCT CCA TG |
hsa-miR-129-5p forward |
CUU UUU GCG GUC UGG GCU UGC |
miRNA Universal reverse |
AAC GCT TCA CGA ATT TGC GT |
U6 forward |
CGC TTC GGC AGC ACA TAT AC |
U6 reverse |
AAA ATA TGG AAC GCT TCA CGA |
For LINC00574 knockdown |
|
Negative control ASO |
CGA CUA TAC GCG CAA UAU GG |
LINC00574 ASO |
GUG ACA GCA AAC TAC CGA AC |
LINC00574 siRNA forward |
GUC CCU CCU GCC UCA GUU UCU CAG A |
LINC00574 siRNA reverse |
UCU GAG AAA CUG AGG CAG GAG GGA CUG |
For luciferase gene reporter assay |
|
LINC00574 PCR forward |
GGG AAA GUT CTC CCA GCC TAG CCC TAC T |
LINC00574 PCR reverse |
GGA GAC AUA AGG GCG AGT AGC AAG AAC A |
For FREMSA |
|
LINC00574 oligo |
GGC AGA GCU CGG GGU UGC UGC GGA GC |
hsa-miR-129-5p oligo |
CUU UUU GCG GUC UGG GCU UGC |
miRNA negative control oligo |
AAC GCT TCA CGA ATT TGC GT |