KEY RESOURCES TABLE
| REAGENT OR RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Biological Samples | ||
| Plasma or serum from rhesus macaques | (Pauthner et al., 2017, Pauthner et al., 2018) | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| BG505 SOSIP.664 | Produced in house (Sanders et al., 2013) | N/A |
| 398F1 SOSIP.664 | Produced in house (deCamp et al., 2014) | N/A |
| 25710 SOSIP.664 | Produced in house (deCamp et al., 2014) | N/A |
| CE1176 SOSIP.664 | Produced in house (deCamp et al., 2014) | N/A |
| X1632 SOSIP.664 | Produced in house (deCamp et al., 2014) | N/A |
| Protein A Resin | GE Healthcare | Cat# 17-5280-02 |
| Superose 6 Increase Size Exclusion Column | GE Healthcare | Cat# 17517201 |
| Uranyl Formate | Electron Microscopy Sciences | Cat# D310 25 GM |
| Lauryl Maltose Neopentyl Glycol | Anatrace | Cat# NG310 5 GM |
| Critical Commercial Assays | ||
| Pierce Fab Preparation Kit | Thermo Fisher Scientific | Cat# 44985 |
| Negative stain EM Grids | Electron Microscopy Sciences | Cat# EMS400-CU |
| Cryo-EM grids | Electron Microscopy Sciences | Cat# Q4100CR2 |
| Deposited Data | ||
| Cryo-EM Reconstruction of Polyclonal Serum Fab from a Protected Rhesus Macaque 4O9 Complexed with BG505 SOSIP.664 | EMDataBank | EMD: 20396 |
| Negative-stain EM map of unimmunized control rhesus macaque BZ05 (challenged with SHIV) serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20399 |
| Negative-stain EM map of unimmunized control rhesus macaque BZ11 (challenged with SHIV) serum fab complexed with BG505 | EMDataBank | EMD: 20400 |
| Negative-stain EM map of unimmunized control rhesus macaque BZ13 (challenged with SHIV) serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20401 |
| Negative-stain EM map of BG505-immunized rhesus macaque 12–060 wk 0 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20402 |
| Negative-stain EM map of BG505-immunized and SHIV-challenged rhesus macaque 12–065 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20403 |
| Negative-stain EM map of BG505-immunized and SHIV-challenged rhesus macaque OQ7 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20404 |
| Negative-stain EM map of BG505-immunized and SHIV-challenged rhesus macaque 12–153 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20405 |
| Negative-stain EM map of BG505-immunized and SHIV-challenged rhesus macaque 12M-248 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20409 |
| Negative-stain EM map of BG505-immunized and SHIV-challenged rhesus macaque 12–149 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20410 |
| Negative-stain EM map of BG505-immunized rhesus macaque 12–143 wk 0 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20415 |
| Negative-stain EM map of BG505-immunized rhesus macaque 12–137 wk 0 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20423 |
| Negative-stain EM map of BG505-immunized rhesus macaque 12M-169 wk 0 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20424 |
| Negative-stain EM map of BG505-immunized rhesus macaque 11M-088 wk 0 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20425 |
| Negative-stain EM map of BG505-immunized rhesus macaque 12–046 wk 0 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20426 |
| Negative-stain EM map of BG505-immunized rhesus macaque 4O9 wk 0 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20427 |
| Negative-stain EM map of BG505-immunized rhesus macaque 12–137 wk 10 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20428 |
| Negative-stain EM map of BG505-immunized rhesus macaque 12–137 wk 28 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20946 |
| Negative-stain EM map of BG505-immunized rhesus macaque 11M-088 wk 12 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20429 |
| Negative-stain EM map of BG505-immunized rhesus macaque 11M-088 wk 28 serum fab complexed with BG505 SOSIP.664 | EMDataBank | EMD: 20970 |
| Negative-stain EM map of BG505-immunized rhesus macaque 4O9 wk 10 serum fab complexed with BG505 SOSIP.664 | EMDataBank | EMD: 20971 |
| Negative-stain EM map of BG505-immunized rhesus macaque 4O9 wk 26 serum fab complexed with BG505 SOSIP.664 | EMDataBank | EMD: 20972 |
| Negative-stain EM map of BG505-immunized rhesus macaque 12–084 wk 26 serum fab complexed with 398F1 SOSIP.664 | EMDataBank | EMD: 20992 |
| Negative-stain EM map of BG505-immunized rhesus macaque 12–137 wk 26 serum fab complexed with 398F1 SOSIP.664 | EMDataBank | EMD: 20936 |
| Negative-stain EM map of BG505-immunized rhesus macaque 12–046 wk 26 serum fab complexed with 398F1 SOSIP.664 | EMDataBank | EMD: 20937 |
| Negative-stain EM map from 3D sorting of unimmunized control rhesus macaque BZ05 (challenged with SHIV) serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20973 |
| Negative-stain EM map from 3D sorting of unimmunized control rhesus macaque BZ05 (challenged with SHIV) serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20974 |
| Negative-stain EM map from 3D sorting of unimmunized control rhesus macaque BZ05 (challenged with SHIV) serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20975 |
| Negative-stain EM map from 3D sorting of unimmunized control rhesus macaque BZ11 (challenged with SHIV) serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20976 |
| Negative-stain EM map from 3D sorting of unimmunized control rhesus macaque BZ11 (challenged with SHIV) serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20977 |
| Negative-stain EM map from 3D sorting of unimmunized control rhesus macaque BZ11 (challenged with SHIV) serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20978 |
| Negative-stain EM map from 3D sorting of unimmunized control rhesus macaque BZ13 (challenged with SHIV) serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20979 |
| Negative-stain EM map from 3D sorting of unimmunized control rhesus macaque BZ13 (challenged with SHIV) serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20980 |
| Negative-stain EM map from 3D sorting of unimmunized control rhesus macaque BZ13 (challenged with SHIV) serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20981 |
| Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 12–060 wk 0 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20982 |
| Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 12–060 wk 0 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20983 |
| Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 12–065 wk 0 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20938 |
| Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 12–065 wk 0 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20984 |
| Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 12–065 wk 0 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20985 |
| Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque OQ7 wk 0 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20939 |
| Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque OQ7 wk 0 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20940 |
| Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 12–143 wk 0 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20941 |
| Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 12–143 wk 0 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20987 |
| Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 12–137 wk 0 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20988 |
| Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 12–137 wk 0 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20989 |
| Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 12–046 wk 0 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20942 |
| Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 12–046 wk 0 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20921 |
| Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 12–046 wk 0 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20943 |
| Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 12–137 wk 10 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20944 |
| Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 12–137 wk 10 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20899 |
| Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 12–137 wk 28 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20898 |
| Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 12–137 wk 28 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20897 |
| Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 12–137 wk 28 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20896 |
| Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 12–137 wk 28 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20896 |
| Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 11M-088 wk 12 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20895 |
| Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 11M-088 wk 12 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20894 |
| Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 11M-088 wk 12 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20893 |
| Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 11M-088 wk 28 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20990 |
| Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 11M-088 wk 28 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20892 |
| Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 11M-088 wk 28 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20891 |
| Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 11M-088 wk 28 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20890 |
| Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 4O9 wk 10 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20889 |
| Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 4O9 wk 10 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20889 |
| Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 4O9 wk 10 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20888 |
| Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 4O9 wk 26 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20887 |
| Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 4O9 wk 26 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20991 |
| Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 4O9 wk 26 serum fab complexed with BG505 SOSIP.664 trimer | EMDataBank | EMD: 20886 |
| Negative-stain EM map of BG505-immunized rhesus macaque 12–046 wk 26 serum fab complexed with 25710 | EMDataBank | EMD: 20885 |
| Negative-stain EM map of BG505-immunized rhesus macaque 12–084 wk 28 serum fab complexed with BG505 SOSIP trimer | EMDataBank | EMD: 20884 |
| PDB Coordinates of BG505 SOSIP.664 trimer complexed with Polyclonal Serum Fab from a Protected Rhesus Macaque 4O9 | Protein Data Bank | PDB ID: 6V0R |
| Experimental Models: Cell Lines | ||
| Human: FreeStyle HEK293F | Thermo Fisher Scientific | Cat# R79007 |
| Human: HEK294T | ATCC | Cat# CRL-3216 |
| Software and Algorithms | ||
| Unicorn 7.0 | GE Healthcare | https://www.gelifesciences.com/ |
| UCSF Chimera | Pettersen et al., 2004 | N/A |
| Appion database | Lander et al., 2009 | N/A |
| Leginon | Suloway et al., 2005 | N/A |
| DoG Picker | Voss et al., 2009 | N/A |
| Relion | Scheres, 2012 | N/A |
| CryoSparc | Punjani et al., 2017 | N/A |