Table 5.
EC50 values from alamarBlue assay in HepG2 2D and 3D cultures after 24 h exposure to TiO2-NPs, Ag-NPs or ZnO-NPs. EC50 values of metal compartments are given in parentheses.
| Substance | 2D | 3D | ||||
|---|---|---|---|---|---|---|
| EC50 (µg/cm2) | EC50 (µg/mL) | EC50 (µM) | EC50 (µg/cm2) | EC50 (µg/mL) | EC50 (µM) | |
| TiO2-NP | >75.0 (>45.0) | >212.1 (>127.1) | >2655.2 | >75.0 (>45.0) | >212.1 (>127.1) | >2655.2 |
| Ag-NP | 3.8 | 10.7 | 99.2 | >30.0 | >84.8 | >786.4 |
| ZnO-NP | 10.1 (8.1) | 28.5 (22.9) | 350.4 | 16.2 (13.0) | 45.7 (36.7) | 561.4 |
| AgNO3 | 1.2 (0.8) | 3.4 (2.2) | 20.1 | - | - | - |
| ZnCl2 | 17.5 (8.4) | 49.4 (23.7) | 362.7 | - | - | - |