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. 2020 Apr 2;29(7):383–400. doi: 10.1089/scd.2019.0150

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© Gowher Ali et al. 2020; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are cited.

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FIG. 2. — Characterization of the generated iPSC lines. Phase contrast of the spontaneously generated EBs from representative Ctr-iPSCs and PsO-iPSCs. Immunostaining showed the expression of the three germ layer markers, NESTIN, BRACHYURY (T), and SOX17 after spontaneous differentiation (A) and direct differentiaiton (B). (C) RT-PCR analysis of the three germ layer markers as indicated. The undifferentiated iPSCs of each sample used as a control. (D) Scorecard analysis of the EBs derived from iPSCs showing a represetnative heatmap of the indicated genes (self-renewal, ectodermal, mesodermal, and endodermal genes). Note that the expression of pluripotency markers were significantly downregulated and the trilineage markers were significantly upregulated in the representative images of Ctr1-iPSCs and PsO2-iPSCs. Scale bars = 100 μm. DW, distal water; EBs, embryoid bodies.