Figure 4.

Chemical blockage of DDR1 phosphorylation modulates receptor downstream signaling in A375, HT29 and SK-HEP cells. Cells were cultured in serum-free media and received exogenous collagen type I. Some cells also received DDR1-IN-1 in a dose correspondent to the IC50 for each cell line. Remained cells were considered controls. (a) Representative Western blot analysis of ICAM1, VCAM1, Ki67, MMP9, phosphorylated AKT (pAKT), total AKT, phosphorylated ERK (pERK) and total ERK expression in the cells in the presence of exogenous collagen I. (b) Computer-assisted semi-quantification of the Western blots for pDDR1/total DDR1, ICAM1, VCAM1, Ki67 and MMP9 expression. (c) Computer-assisted semi-quantification of the Western blots for pAKT/total AKT and pERK/total ERK expression in the cell (results are presented as the ratio phosphorylated form/total protein). Data are presented as the means ± standard error, n = 3 (*P < 0.05, **P < 0.001, ***P < 0.0001).