Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Mar 22;10(3):581. doi: 10.3390/nano10030581

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Expression of αvβ3 by HUVECs under different conditions (a) Flow cytometry analysis demonstrated significant expression of integrin αvβ3 in overnight starved cells. (i) Side scatter (SSC) vs. forward scatter (FSC) plot used to gate live cells and resulting (ii) histograms from which αvβ3 expression was (iii) quantified for different culture conditions. Colors in (ii) match the conditions shown in the x-axis of (iii). Values were expressed as mean ± SD, n = 3. p < 0.005 was considered significant. (b) Immunostaining was performed by (i) staining HUVECs with anti-human CD51/CD61 antibody to detect integrin αvβ3 (green). Nuclei were stained by DAPI (blue). Integrin αvβ3 expression was highest under overnight starved conditions when compared to control and 2 h starvation. (ii) Quantification of the images in (i) obtained using Image J. Value was expressed as mean ± SD, n = 3. p < 0.05 was considered significant.