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. 2020 Mar 10;4(4):bvaa028. doi: 10.1210/jendso/bvaa028

Table 1.

Nucleotide Sequences for Quantitative Polymerase Chain Reaction Primers

Gene qPCR Sequences 5ʹ to 3ʹ
18S F: CGGCTACCACATCCAAGGAA R: GCTGGAATTACCGCGGCT
Runx2 F: CCAACCGAGTCATTTAAGGCT R: GCTCACGTCGCTCATCTTG
Osx F: GGAAAGGAGGCACAAAGAAGC R: CCCCTTAGGCACTAGGAG
Alp F: CCAACTCTTTTGTGCCAGAGA R: GGCTACATTGGTGTTGAGCTTTT
Col1a1 F: TGCTTCCACGTTTACAGCTCTAAAG R: GTCAGGAAAGGGTCATCTGTAGTCC
Ocn F: TGCTGGAGTGGTCTCTATGA R: ACCCTCTTCCCACACTGT
Atf4 F: GCATGCTCTGTTTCGAATGGA R: CCAACGTGGTCAAGAGCTCAT
Tars F: CCCTGGCCTGAATACATTAACAC R: CGGCTTGCTATCTTTTGCTGC
Asns F: CAAGGAGCCCAAGTTCAGTAT R: GGCTGTCCTCCAGCCAAT
NFATc1 F: GGTAACTCTGTCTTTCTAACCTTAAGCTC R: GTGATGACCCCAGCATGCACCAGTCACAG
CatK F: GAAGAAGACTCACCAGAAGCAG R: TCCAGGTTATGGGCAGAGATT
Cox2 F: GCCGACTAAATCAAGCAACA R: CAATGGGCATAAAGCTATGG
Cox5 F: GGGTCACACGAGACAGATGA R: GGAACCAGATCATAGCCAACA
ATP5γ1 F: AGTTGGTGTGGCTGGATCA R: GCTGCTTGAGAGATGGGTTC
Idh3a F: CCTCCTGCTTAGTGCTGTGA R: CGTTGCCTCCCAGATCTTT

Abbreviation: qPCR, quantitative polymerase chain reaction.