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. Author manuscript; available in PMC: 2020 Apr 13.
Published in final edited form as: J Biol Rhythms. 2019 Apr 17;34(3):249–271. doi: 10.1177/0748730419841468

Table 1.

DD Period and Rhythm Strength Following Clock Cell Manipulations. Full genotype, abbreviated name, clock cell expression, number of animals, mean period length ± SEM and mean rhythm power ± SEM are listed for each clock cell manipulation. Mean rhythm power for each experimental line was compared to corresponding GAL4 and UAS controls with ANOVA followed by Tukey HSD posthoc test. Italicized lines are significantly different from both genetic controls at p<0.05.

Genotype Name Clock Cell Expression n Period ± SEM Power ± SEM Tukey HSD p-values
----cycDN experiments----
 UAS-cycDN/+  +>cycDN  --  299  23.34 ± 0.04  1847.8 ± 43.2  
 Clk856-GAL4/UAS-cycDN  Clk856>cycDN  All clock cells except some DN3s  66  24.88 ± 0.60  144.2 ± 22.0****  to cycDN: <1.0E-15 to GAL4: 2.0E-14
 Clk856-GAL4/+  Clk856>+  --  59  23.62 ± 0.03  953.6 ± 54.7  
 Pdf-GAL4/UAS-cycDN  pdf>cycDN  LNvs  91  23.91 ± 0.30  320.4 ± 28.9****  to cycDN: 4.4E-14 to GAL4: 5.3E-14
 Pdf-GAL4/+  pdf>+  --  80  23.93 ± .010  1317.5 ± 70.7  
 Clk856-GAL4/UAS-cycDN; Pdf- GAL80/+  Clk856(Pdf80)> cycDN  All clock cells except LNvs and some DN3s  63  23.77 ± .018  557.2 ± 51.9****  to cycDN: <1.0E-15 to GAL4: 3.1E-09
 CLK856-GAL4/+; Pdf-GAL80/+  Clk856(Pdf80)>+  --  67  23.64 ± 0.03  1267.6 ± 77.3  
 MB122B-AD/UAS-cycDN; MB122-DBD/+  MB122B>cycDN  3 CRY+ LNds; 5th sLNv  78  23.36 ± 0.04  722.9 ± 46.4  to cycDN: 1.6E-13 to GAL4: 0.203
 MB122-AD/+; MB122-DBD/+  MB122B>+  --  79  23.56 ± 0.09  874.0 ± 47.3  
 UAS-cycDN/+; R78G02-GAL4/+  R78G02>cycDN  3 CRY+ LNds; 5th sLNv  52  23.50 ± 0.09  1229.9 ± 107.1  to cycDN: 1.5E-3 to GAL4: 0.387
 R78G02-GAL4/+  R78G02>+  --  54  23.72 ± 0.03  1417.3 ± 95.3  
 UAS-cycDN/+; Clk4.1-GAL4/+  Clk4.1>cycDN  8–10 DN1p  62  23.83 ± 0.04  1158.0 ± 92.5  to cycDN: 7.5E-05 to GAL4: 0.114
 Clk4.1-GAL4/+  Clk4.1>+  --  59  23.69 ± 0.05  1419.0 ± 82.1  
 UAS-cycDN/+; InSITE911-GAL4/+  InSITE911>cycDN  All DN1ps  47  23.66 ± 0.05  1574.8 ± 110.0  to cycDN: 0.111 to GAL4: 0.991
 +/+; InSITE911-GAL4/+  InSITE911>+  --  48  23.76 ± 0.04  1594.5 ± 105.8  
 Clk9M-GAL4/ UAS-cycDN; Pdf- GAL80/+  Clk9M(Pfd80) >cycDN  DN2s  18  23.31 ± 0.07  1568.3 ± 144.8  to cycDN: 1.31E-2 to GAL4: 0.983
 Clk9M-GAL4/+; Pdf-GAL80/+  Clk9M(Pdf80)>+  --  31  23.45 ± 0.06  1534.5 ± 113.8  
 UAS-cycDN/+; Pdf-GAL80/+  +(Pdf80)>cycDN  --  133  23.57 ± 0.02  1729.4 ± 59.7  
 UAS-cycDN/+; R78G02-GAL4,Pdf-GAL80/Pdf-GAL80  R78G02(Pdf802)> cycDN  3 CRY+ LNds; 5th sLNv  61  23.62 ± 0.04  760.8 ± 69.5  to cycDN: <1.0E-15 to GAL4: 0.703
 R78G02-GAL4,Pdf-GAL80/Pdf-GAL80  R78G02(Pdf802)> +  --  64  23.65 ± 0.11  849.5 ± 66.3  
 DvPdf-GAL4,Pdf-GAL80/UAS-cycDN; Pdf-GAL80/Pdf-GAL80  DvPdf(Pdf803)> cycDN  3 CRY- LNds; 1 CRY+ LNd; 5th sLNv  53  24.32 ± 0.17  750.3 ± 79.6  to cycDN: <1.0E-15 to GAL4: 0.831
 DvPdf-GAL4,Pdf-GAL80/+; Pdf-GAL80/Pdf-GAL80  DvPdf(Pdf803)>+  --  63  23.87 ± 0.05  817.3 ± 61.7  
 DvPdf-GAL4,Pdf-GAL80/UAS-cycDN; R78G02-GAL4,Pdf- GAL80/Pdf-GAL80  LNd>cycDN  All LNds; 5th sLNv  64  23.45 ± 0.12  543.5 ± 43.1****  to cycDN: 9.7E-14 to GAL4: 4.7E-06
 Dvpdf-GAL4,pdf-GAL80/+; R78G02-GAL4,pdf-GAL80/Pdf-GAL80  LNd>+  --  63  23.67 ± 0.03  1085.5 ± 84.6  
 DvPdf-GAL4/UAS-cycDN; Pdf-GAL80/+  DvPdf(Pdf80)> cycDN  Four LNds; LNvs  48  23.83 ± 0.32  464.5 ± 60.5****  to cycDN: 2.0E-15 to GAL4: 8.1E-11
 Dvpdf-GAL4/+; Pdf-GAL80/+  DvPdf(Pdf80)>+  --  47  24.26 ± 0.05  1329.9 ± 100.6  
 DvPdf-GAL4,Pdf-GAL80/UAS-cycDN; Pdf-GAL80/+  DvPdf(Pdf802)> cycDN  Four LNds; LNvs  38  23.49 ± 0.40  422.3 ± 73.4****  to cycDN: 8.1E-14 to GAL4: 8.6E-07
 DvPdf-GAL4,Pdf-GAL80/+; Pdf-GAL80/+  DvPdf(Pdf802)>+  --  43  24.01 ± 0.05  1197.3 ± 106.3  
----Kir2.1 experiments----
 UAS-Kir2.1/+  +>Kir2.1  --  108  23.71 ± 0.04  1102.7 ± 59.1  
 Clk856-GAL4/+; UAS-Kir2.1/+  Clk856>Kir2.1  All clock cells except some DN3s  50  25.30 ± 0.61  153.5 ± 8.7****  to Kir2.1: <1.0E-15 to GAL4: 5.0E-15
 Clk856-GAL4/+  Clk856>+  --  40  23.68 ± 0.05  1055.8 ± 101.9  
 Pdf-GAL4/+; UAS-Kir2.1/+  Pdf>Kir2.1  LNvs  62  22.84 ± 0.41  146.0 ± 9.9****  to Kir2.1: 1.5E-14 to GAL4: 5.0E-15
 Pdf-GAL4/+  Pdf>+  --  64  23.95 ± 0.06  1300.1 ± 76.2  
 Clk856-GAL4/+; UAS-Kir2.1/Pdf-GAL80  Clk856(Pdf80)> Kir2.1  All clock cells except LNvs and some DN3s  63  24.87 ± 0.38  155.2 ± 10.3****  to Kir2.1: <1.0E-15 to GAL4: <1.0E-15
 Clk856-GAL4/+; Pdf-GAL80/+  Clk856(Pdf80)>+  --  67  23.64 ± 0.03  1267.6 ± 77.3  
 MB122B-AD/+; MB122B-DBD/UAS-Kir2.1  MB122B>Kir2.1  3 CRY+ LNds; 5th sLNv  91  24.16 ± 0.13  406.5 ± 27.1****  to Kir2.1: 3.3E-13 to GAL4: 3.5E-13
 MB122B-AD/+; MB122B-DBD/+  MB122B>+  --  88  23.61 ± 0.02  1018.3 ± 51.8  
 UAS-Kir2.1,Pdf-GAL80/+  +(Pdf80)>Kir2.1  --  92  23.95 ± 0.02  1090.4 ± 56.4  
 DvPdf-GAL4,Pdf-GAL80/+; UAS-Kir2.1,Pdf-GAL80/Pdf-GAL80  DvPdf(Pdf803)> Kir2.1  3 CRY- LNds; 1 CRY+ LNd; 5th sLNv  71  24.49 ± 0.09  563.7 ± 47.8***  to Kir2.1: 3.5E-09 to GAL4: 1.9E-04
 DvPdf-GAL4,Pdf-GAL80/+; Pdf-GAL80  DvPdf(Pdf803)>+  --  90  23.82 ± 0.03  903.6 ± 62.7  
----tubGAL80ts; Kir2.1 experiments----
 tub-GAL80ts/+; UAS-Kir2.1/+ 18°C  +>Kir2.1ts  --  53  23.73 ± 0.14  864.7 ± 56.3  
 Clk856-GAL4/tub-GAL80ts; UAS-Kir2.1/Pdf-GAL80 18°C  Clk856(Pdf80)> Kir2.1ts  All clock cells except LNvs and some DN3s  67  23.74 ± 0.17  877.9 ± 61.9  to Kir2.1: 0.999 to GAL4: 0.159
 Clk856-GAL4/+; Pdf-GAL80/+ 18°C  Clk856(Pdf80)>+  --  53  23.61 ± 0.10  646.9 ± 39.6  
 MB122B-AD/tub-GAL80ts; MB122B-DBD/UAS-Kir2.1 18°C  MB122B>Kir2.1ts  3 CRY+ LNds; 5th sLNv  42  24.18 ± 0.24  402.3 ± 33.9  to Kir2.1: 4.3E-04 to GAL4: 0.998
 MB122B-AD/+; MB122B-DBD/+ 18°C  MB122B>+  --  59  23.67 ± 0.09  449.6 ± 25.0  
 tub-GAL80ts/+; UAS-Kir2.1/+ 30°C  +>Kir2.1ts  --  58  23.86 ± 0.07  1985.2 ± 103.1  
 Clk856-GAL4/tub-GAL80ts; UAS-Kir2.1/Pdf-GAL80 30°C  Clk856(Pdf80)> Kir2.1ts  All clock cells except LNvs and some DN3s  79  22.45 ± 0.44  37.6 ± 6.0****  to Kir2.1: <1.0E-15 to GAL4: <1.0E-15
 Clk856-GAL4/+; Pdf-GAL80/+ 30°C  Clk856(Pdf80)>+  --  58  23.38 ± 0.03  2713.1 ± 101.5  
 MB122B-AD/tub-GAL80ts; MB122B-DBD/UAS-Kir2.1 30°C  MB122B>Kir2.1ts  3 CRY+ LNds; 5th sLNv  41  23.79 ± 0.14  1363.7 ± 97.0****  to Kir2.1: 2.9E-07 to GAL4: 1.0E-11
 MB122B-AD/+; MB122B-DBD/+ 30°C  MB122B>+  --  60  23.62 ± 0.03  2398.9 ± 79.2  
****

p<0.0001

***

p<0.001 compared to both genetic controls. Specific Tukey HSD p-values are listed.